稻米垩白性状对高温耐性的QTL分析

doi:10.16819/j.1001-7216.2017.6172 257

摘要/Abstract

摘要：

【目的】本研究旨在筛选与稻米外观品质高温耐性连锁的分子标记,为稻米品质育种提供参考。【方法】以耐热水稻品系996和热敏感水稻品系4628为亲本构建的重组自交系为材料,采用垩白粒率耐热指数、垩白大小耐热指数和垩白度耐热指数为评价指标,对水稻垩白性状的高温耐性QTL进行检测。【结果】采用复合区间作图法两年共检测到垩白性状高温耐性QTL 24个,包括垩白粒率高温耐性QTL 8个,垩白大小高温耐性QTL 12个,垩白度高温耐性QTL 4个。其中,第6染色体上的2个垩白粒率高温耐性QTL和第7染色体上的2个垩白度高温耐性QTL在两年中重复检测到,且这2个稳定表达的垩白度位点与2015年检测到的第7染色体上的垩白粒率位点重合。另外,发现有4个QTL一因多效,同时影响垩白粒率、垩白大小及垩白度。【结论】控制垩白粒率耐热指数的qHTCGR6.1和控制垩白度耐热指数的qHTCD7.1是新的QTL。

关键词: 稻米, 垩白性状, 高温耐性, 数量性状基因座位

Abstract:

【Objective】The molecular markers linked to high temperature tolerance of rice appearance quality were screened for the purpose of providing an important foundation of improvement for rice grain quality. 【Method】The quantitative trait loci (QTLs) for heat tolerance of grain chalkiness trait were identified using the recombinant inbred lines(RILs) derived from a cross between the heat tolerant rice line 996 and the heat sensitive line 4628. The heat tolerance indexes of chalky grain rate, chalkiness size and chalkiness degree were used as the heat-tolerance indicators. 【Result】24 QTLs for heat tolerance of grain chalky traits were detected in two years by using composite interval mapping analysis including eight for heat tolerance of chalky grain rate(HTCGR), twelve for heat tolerance of chalkiness size and fourteen for heat tolerance of chalkiness degree(HTCD). Two HTCGR QTLs on chromosome 6 and two HTCD QTLs on chromosome 7 could be detected repeatedly in two years. The two HTCD sites coincided with two HTCGR sites on chromosome 7 detected in 2015. Moreover, anyone of the four pleiotropic QTLs had impacts on the chalky grain rate, chalkiness size and chalkiness degree. 【Conclusion】The QTL controlling the heat tolerance index of chalkiness degree qHTCGR6.1 and the QTL controlling the heat tolerance of chalkiness degree qHTCD7.1 might be new findings.．

Key words: rice, chalkiness trait, thermo-tolerance, quantitative trait locus

中图分类号:

张桂莲, 廖斌, 唐文帮, 陈立云, 肖应辉. 稻米垩白性状对高温耐性的QTL分析[J]. 中国水稻科学, 2017, 31(3): 257-264.

Guilian ZHANG, Bin LIAO, Wenbang TANG, Liyun CHEN, Yinghui XIAO. Identifying QTLs for Thermo-tolerance of Grain Chalkiness Trait in Rice[J]. Chinese Journal OF Rice Science, 2017, 31(3): 257-264.

图/表 4

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性状 Trait	年份 Year	亲本 Parent
性状 Trait	年份 Year	996	4628		平均值Mean		变幅Range
垩白粒率耐热指数 Heat tolerance index of chalky grain rate	2014	30.65±1.07	70.25±1.76	55.11±1.65		2.54~84.56
垩白粒率耐热指数 Heat tolerance index of chalky grain rate	2015	31.45±0.66	75.68±2.27	56.12±1.96		0.00~90.91
垩白大小耐热指数 Heat tolerance index of chalkiness size	2014	40.78±1.43	65.78±1.64	42.29±1.23		11.3~65.53
垩白大小耐热指数 Heat tolerance index of chalkiness size	2015	41.12±1.53	70.14±2.10	44.11±1.54		2.76~95.23
垩白度耐热指数 Heat tolerance index of chalkiness degree	2014	40.43±1.54	67.53±2.03	48.82±1.71		5.03~72.70
垩白度耐热指数 Heat tolerance index of chalkiness degree	2015	41.39±1.45	73.42±2.57	50.77±1.78		7.66~91.47

性状 Trait	年份 Year	亲本 Parent
性状 Trait	年份 Year	996	4628		平均值Mean		变幅Range
垩白粒率耐热指数 Heat tolerance index of chalky grain rate	2014	30.65±1.07	70.25±1.76	55.11±1.65		2.54~84.56
垩白粒率耐热指数 Heat tolerance index of chalky grain rate	2015	31.45±0.66	75.68±2.27	56.12±1.96		0.00~90.91
垩白大小耐热指数 Heat tolerance index of chalkiness size	2014	40.78±1.43	65.78±1.64	42.29±1.23		11.3~65.53
垩白大小耐热指数 Heat tolerance index of chalkiness size	2015	41.12±1.53	70.14±2.10	44.11±1.54		2.76~95.23
垩白度耐热指数 Heat tolerance index of chalkiness degree	2014	40.43±1.54	67.53±2.03	48.82±1.71		5.03~72.70
垩白度耐热指数 Heat tolerance index of chalkiness degree	2015	41.39±1.45	73.42±2.57	50.77±1.78		7.66~91.47

染色体 Chromosome	位点 QTL	标记区间 Marker interval		LOD值 LOD score		加性效应 Additive effect		贡献率 Phenotypic variations explained/%
染色体 Chromosome	位点 QTL	标记区间 Marker interval		2014	2015	2014	2015	2014	2015
垩白粒率高温耐性Heat tolerance of chalky grain rate (HTCGR)
1	qHTCGR1.1	RM297	–RM6648	3.50		0.04		9.04
1	qHTCGR1.2	RM6648	–RM6387	3.48		0.04		8.48
3	qHTCGR3	SFP3_1	–RM231	3.30		0.04		7.91
6	qHTCGR6.1	RM3353	–RM1369	4.44	7.24	0.06	0.03	14.24	12.14
6	qHTCGR6.2	RM1369	–RM190	4.78	7.47	0.06	0.04	17.89	14.25
7	qHTCGR7.1	RM3859		3.06		0.03		7.98
7	qHTCGR7.2	RM21327	–RM21364		3.56		0.06		10.30
7	qHTCGR7.3	RM21364	–RM3859		3.44		0.05		7.66
垩白大小高温耐性Heat tolerance of chalkiness size (HTCS)
1	qHTCS1	RM8003	–RM237	3.19		–0.10		15.08
2	qHTCS2.1	RM7245	–RM6366	3.99		0.17		10.90
2	qHTCS2.2	RM6366	–RM1367	3.75		0.16		10.31
4	qHTCS4	RM3866			2.90		0.16		7.35
6	qHTCS6.1	RM3353	–RM1369		2.76		–0.15		6.91
6	qHTCS6.2	RM1369	–RM190		2.65		–0.19		10.74
7	qHTCS7.1	RM21327	–RM21364		3.03		–0.17		7.86
7	qHTCS7.2	RM21364	–RM3859		4.02		–0.25		18.76
10	qHTCS10.1	RM6100	–RM25678	4.07		–0.14		11.60
10	qHTCS10.2	RM25678	–RM6745	3.69		0.14		10.58
10	qHTCS10.3	RM6745	–RM25681	4.00		–0.14		11.40
10	qHTCS10.4	RM25681	–RM7300	3.02		–0.13		9.68
垩白度高温耐性 Heat tolerance of chalkiness degree (HTCD)
1	qHTCD1	RM8003	–RM237	2.98		–0.10		11.54
3	qHTCD3	RM6349	–SFP31	4.81		–0.17		36.34
7	qHTCD7.1	RM21327	–RM21364	4.68	5.54	–0.15	–0.14	13.27	17.44
7	qHTCD7.2	RM21364	–RM3859	4.25	6.25	–0.16	–0.17	16.32	29.52

染色体 Chromosome	位点 QTL	标记区间 Marker interval		LOD值 LOD score		加性效应 Additive effect		贡献率 Phenotypic variations explained/%
染色体 Chromosome	位点 QTL	标记区间 Marker interval		2014	2015	2014	2015	2014	2015
垩白粒率高温耐性Heat tolerance of chalky grain rate (HTCGR)
1	qHTCGR1.1	RM297	–RM6648	3.50		0.04		9.04
1	qHTCGR1.2	RM6648	–RM6387	3.48		0.04		8.48
3	qHTCGR3	SFP3_1	–RM231	3.30		0.04		7.91
6	qHTCGR6.1	RM3353	–RM1369	4.44	7.24	0.06	0.03	14.24	12.14
6	qHTCGR6.2	RM1369	–RM190	4.78	7.47	0.06	0.04	17.89	14.25
7	qHTCGR7.1	RM3859		3.06		0.03		7.98
7	qHTCGR7.2	RM21327	–RM21364		3.56		0.06		10.30
7	qHTCGR7.3	RM21364	–RM3859		3.44		0.05		7.66
垩白大小高温耐性Heat tolerance of chalkiness size (HTCS)
1	qHTCS1	RM8003	–RM237	3.19		–0.10		15.08
2	qHTCS2.1	RM7245	–RM6366	3.99		0.17		10.90
2	qHTCS2.2	RM6366	–RM1367	3.75		0.16		10.31
4	qHTCS4	RM3866			2.90		0.16		7.35
6	qHTCS6.1	RM3353	–RM1369		2.76		–0.15		6.91
6	qHTCS6.2	RM1369	–RM190		2.65		–0.19		10.74
7	qHTCS7.1	RM21327	–RM21364		3.03		–0.17		7.86
7	qHTCS7.2	RM21364	–RM3859		4.02		–0.25		18.76
10	qHTCS10.1	RM6100	–RM25678	4.07		–0.14		11.60
10	qHTCS10.2	RM25678	–RM6745	3.69		0.14		10.58
10	qHTCS10.3	RM6745	–RM25681	4.00		–0.14		11.40
10	qHTCS10.4	RM25681	–RM7300	3.02		–0.13		9.68
垩白度高温耐性 Heat tolerance of chalkiness degree (HTCD)
1	qHTCD1	RM8003	–RM237	2.98		–0.10		11.54
3	qHTCD3	RM6349	–SFP31	4.81		–0.17		36.34
7	qHTCD7.1	RM21327	–RM21364	4.68	5.54	–0.15	–0.14	13.27	17.44
7	qHTCD7.2	RM21364	–RM3859	4.25	6.25	–0.16	–0.17	16.32	29.52